0  296269  296277  296283  296287  296293  296295  296299  296305  296307  296313  296319  296323  296325  296329  296335  296337  296343  296347  296349  296353  296355  296359  296361  296363  296364  296365  296367  296368  296369  296371  296373  296377  296379  296383  296385  296389  296395  296397  296403  296407  296409  296413  296419  296425  296427  296433  296437  296439  296445  296449  296455  296463  447090 

21. She _______ me ______ her parents as I met them for the first time.

A. showed; to    B. drove; to    C. brought; to    D. introduced; to

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20. Only by hard working can you _______ the goal.

A. achieve       B. receive     C. make      D. take

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19. This kind of exercise is most _______, and therefore is a good test of your students’ ability.

A. surprising        B. encouraging   C. exciting    D. challenging

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18. For the young, ______ should be showed to their parents, teachers and old people.

A. respect    B. seriousness   C. laugh      D. laziness

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17. If most breadwinners ________ a day’s pay to the Hope Project, then it will be hopeful.

A. gift       B. donate     C. present     D  give

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16. His parents wouldn’t let him marry anyone ______ family was poor.

A.of whom B.whom   C.of whose   D.whose 

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Section A

Directions: In this section you’ll hear 6 conversations between 2 speakers. For each conversation, there are several questions and each question is followed by 3 choices. Listen to the conversations carefully and then answer the questions by marking the corresponding letter (A, B or C) on the question booklet.

You will hear each conversation TWICE.

Conversation 1

1. What does the woman advise the man to do?

    A. Study hard       B. See a doctor      C. Take a break

2. What’s the result of their last game?

    A. The man won     B. The woman won     C. Both played badly

Conversation 2

3. How long has the woman waited for Mr. Brown’s reply?

    A. About a month     B. About half a month     C. About a week

4. Why did the woman send Mr. Brown an e-mail?

A.   To see if he’d like to come for the lecture. 

B.    To ask for a day off.

C.   To make sure when he will be back

Conversation 3

5. Why is Jimmy staying at a Youth Hostel?

A.   He’s attending a meeting there.   B. He’s a member of the YHA.

C. The Youth Hostel has excellent service.

6. What do we know about Jack’s mother?

A.   Something may be wrong with her ears.

B.    She asked Jimmy to write letters back.

C.   She ‘d rather speak to Jimmy on phone.

Conversation 4

7. Where does the conversation take place?

    A. At the history research center     B, At a library

    C. In the woman’s office

8. What has happened to the yearbook?

    A. It’s lost.     B. The man left it home.    C. The woman’s friend keeps it now.

9. What can we infer from the conversation?

A.   The man is working for the history research center.

B.    The woman is angry with the man’s blaming.

C.   One can’t keep a book as long as three months.

Conversation 5

10. Why hasn’t the woman seen the man for some time?

A.   He has been on vacation.

B.    He was catching up on things at home.

C.   He has been sick.

11. What is the relationship between the two speakers?

    A. Co-workers.     B. Classmates       C. Cousins

12. What can we learn about the woman from the dialogue?

A.   She was taking care of her mother who had the flu.

B.    She has been sick.

C.   She didn’t have the flu.

Conversation 6

13. What’s the relationship between the two speakers?

   A. Boss and secretary     B. Wife and husband    C. Teacher and student

14. Where does the conversation probably take place?

   A. In the office       B. At  home       C. In the classroom

15. Why does the woman repeat the man’s words “ housework is different”?

   A. Because she is angry.    B. Because she is puzzled. 

 C. Because she is pleased.

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125、     C/N=4/1   菌体大量繁殖但产生的谷氨酸少(P79)

    记住  C/N=3/1   菌体繁殖受抑制,但谷氨酸的合成量大增

       溶氧不足:  产生乳酸或琥珀酸

       pH呈酸性: 产生乙酰谷氨酰胺(P95)

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124、发酵工程   内容  灭菌:去除杂菌

               扩大培养和接种:菌种多次培养达到一定数量

             发酵过程:(中心阶段)控制各种条件,生产发酵产品

         分离提纯  菌体:过滤、沉淀(单细胞蛋白即微生物菌体本身)

               代谢产物:蒸馏、萃取、离子交换

         应用  医药工业:生产药品和基因工程药品

             食品工业:传统发酵产品、食品添加剂、单细胞蛋白等

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123、高压蒸汽灭菌法:1/5、1/2、2/3、75%  由里向外、细密、不重复

   溶化后分装前必须要 调节pH 

   细菌培养的过程:培养基的配制→灭菌→搁置斜面→接种→培养观察

          实例:谷氨酸发酵(黄色短杆菌、谷氨酸棒状杆菌)

           概念:

               菌种选育:诱变育种、基因工程、细胞工程

               培养基的配制:成分、比例,pH适宜

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