0  355554  355562  355568  355572  355578  355580  355584  355590  355592  355598  355604  355608  355610  355614  355620  355622  355628  355632  355634  355638  355640  355644  355646  355648  355649  355650  355652  355653  355654  355656  355658  355662  355664  355668  355670  355674  355680  355682  355688  355692  355694  355698  355704  355710  355712  355718  355722  355724  355730  355734  355740  355748  447090 

26.It would be unwise to      too much significance      these differences.

   A. attach; to        B. refer; to              C. apply; to         D. contribute; to

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25.Most female workers in Shanghai suffer from      connected to the global financial crisis, according to a recent survey.

A. curiosity          B. eagerness             C. anxiety           D. thirst

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24.- Why was Professor Liu thundering in the classroom yesterday?

- A student’s interrupting his speech      the burst of his anger.

A. set up           B. set out               C. set about         D. set off

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23.- Sunny day, isn’t it?    

- Let’s hope the sunny weather      for Saturday’s tennis match.

A. carries on         B. moves on             C. keeps up         D. goes up

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22.The rich only      themselves in making money and had no eye for the needs and welfare of their workers

A. devoted         B. concerned        C. enjoyed          D. occupied

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第一节:单项选择(共10小题;每小题1分,满分10分)

从A、B、C、D四个选项中,选出可以填入空白处的最佳选项,并在答题卡上将该项涂黑。

21.Many middle school teachers are too heavily      with teaching to engage in research work.

    A. burdened         B. related             C. associated       D. provided

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1. What is Agatha Christie?

A. A writer.          B. A bookseller.         C. A detective.

2. What time is it now?

A. 8:00.              B. 7:45.            C. 8:15.

3. Where are the two speakers?

A. Outside a theatre.          B. In a bank.           C.  At a show.

4. How did the man like the beef?

A. Delicious.            B. Satisfying.          C. Not satisfying.

5. What is the man doing?

A. Taking advice.                       B. Making a suggestion.   

C. Asking for permission.

六(6-7)

6. What does the man think the weather is going to be like tomorrow?

A. Rainy.             B. Cloudy.           C. Sunny.

7. What will the woman probably do tomorrow?

A. Go to the picnic with an umbrella.         B. Stay home because of the rain.

C. Make sure of the weather.

七(8-9)

8. How much money was there in the purse?

A. About $15.            B. About $ 50.           C. About $ 55.

9. Where did the woman most probably leave her purse?

A. In the street.         B. In the coffee shop.          C. In the shoe department.

八(10-11)

10. Why does the woman look pale?

A.  She is probably sick.                    B. She feels a bit tired of her work.

C. She works too hard with her new students.

11. What is the relationship between the two speakers?

A. Doctor and patient.     B. Headmaster and teacher.      C. Teacher and student.

九(12-14)

12. What is the woman asking about?

A. How to earn money.                      B. How to walk dogs. 

C. How to make advertisements.

13. What is the man's first suggestion?

A. Walking dogs.      B. Buying a bike.       C. Carrying papers.

14. Why does the woman prefer the job of walking dogs?

A. She doesn't have a bike.                B. She can make more money.

C.  It's good for her health.

十(15-17)

15. When did the story happen?

A. Early in the morning.            B. Late in the afternoon.       C. At midnight.

16. What happened to the woman finally?

A. She was killed.     B. She was saved.   C. She was pulled into the water.

17. What kind of person is the woman?

A. Friendly.            B. Quick.          C.  Brave.

十一(18-20)

18. What does the speaker mainly talk about?

A. The university library.            B. The library rules.           C. The library hours.

19. Where is the most likely place to find a book on physics?

A. Level 1.            B. Level 2.          C. Level 3.

20. How long can a graduate student keep the borrowed books?

A. 14 days.              B. 20 days.           C. 28 days.

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43.(11分) 味精是家庭不可缺少的调味品,它的主要成分谷氨酸钠,是将谷氨酸用碳酸钠中和而成。目前主要利用谷氨酸棒状杆菌来生产谷氨酸。下图表示某容器中培养的谷氨酸棒状杆菌的生长曲线。

请回答:

(1)谷氨酸棒状杆菌的代谢类型是_______________________。

(2)为了测定培养液中细菌的数目,将500个红细胞与5mL该培养液混合均匀,然后制片观察,进行随机统计结果如下:

 
   视野l
   视野2
   视野3
   视野4
  红细胞数
   20
   18
   22
   20
  细菌个数
   103
   97
   105
   95

 该5mL培养液共含有细菌约_________________个。

(3)当培养基中的碳源和氮源之比为4:1时,有利于图中的__________段进行。如果想推迟de段的出现,可以采用_________________的方法。

(4)对照图a,在图b绘出表示谷氨酸棒状杆菌生长速率变化的曲线。

(5)在谷氨酸的生产过程中,采用一定的手段改变细胞膜的透性的目  

(6)当培养基中____________不足时,发酵罐中有乳酸或琥珀酸出现。

(7)为测定谷氨酸棒状杆菌生长所需的最适pH,某同学设计了下列实验步骤。

   配制适宜培养基,分装到5个锥形瓶中,并编号I、Ⅱ、Ⅲ、Ⅳ、V;用缓冲液分别调pH为4.0、4.5、5.O、5.5、6.O;将每瓶培养基等量分装到3支试管中,加棉塞并用牛皮纸包扎,每捆试管外挂上相应标签;在酒精灯上加热煮沸10min灭菌后冷却;无菌条件下接种等量菌种;37℃恒温箱中培养24小时;测定每管中谷氨酸的产量;计量、统计并分析结果。

A.上述步骤中有3个明显科学性错误,请改正之:

①_____________________________________________________________。

②_____________________________________________________________。

③_____________________________________________________________。

B.试列出实验结果的数据统计表格(含标题)

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42.(13分) 1965年我国科学工作者人工合成了具有全部生物活性的结晶牛胰岛素。此物质与天然的牛胰岛素是否是同一种物质?科学家做了如下的检验:在人工合成牛胰岛素过程中,掺入放射性14C,然后把掺入14C的人工合成的牛胰岛素与天然的牛胰岛素混合在一起。经过多次重新结晶后,得到了放射性14C分布均匀的牛胰岛素。请据此回答下列问题:

   (1)合成牛胰岛素所需要的原料是       ,此原料彼此连接的化学反应是      .胰岛素的两条肽链(21肽和30肽)再形成一定的         结构,才具有胰岛素的性质和作用。

   (2)若上述原料的平均分子量是P的话,合成的牛胰岛素的分子量是            

(3)在检验人工合成牛胰岛素的过程中,掺入的放射性C是作为       ,这种方法称为  

    法。

   (4)天然的牛胰岛素是在牛的胰岛细胞内合成的。在胰岛细胞内控制胰岛素合成的基因中,至少应有脱氧核苷酸       个。若核糖核苷酸的平均分子量是Q的话,那么与牛胰岛素合成有关的遗传密码子链的分子量约为              

   (5)在检验人工合成的牛胰岛素的过程中,得到了C分布均匀的牛胰岛素结晶,这说明                               

(6)人工合成的牛胰岛素具有全部的生物活性,主要表现在它能够促进       的分解和促进      的合成。前者的化学反应式是:                 。因此胰岛素的主要生理作用是                         

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41.(8分) 某实验小组为了探究细胞膜的通透性,将小鼠肝细胞在体外培养一段时间后,检测培养液中的氨基酸、葡萄糖和尿素含量,发现它们的含量发生了明显的变化(如下图)。请回答问题。

(1)由图可知,随培养时间延长,培养液中葡萄糖和氨基酸含量       ,尿素含量       。由于在原培养液中没有尿素,推测其是      的产物。

(2)培养液中的氨基酸进入细胞后,其主要作用是      ;被吸收的葡萄糖主要通过      作用,为细胞提供    

(3)转氨酶是肝细胞内参与氨基酸分解与合成的一类酶,正常情况下这类酶不会排出胞外,若在细胞培养液中检测到该类酶,可能的原因是    

(4)由(1)和(3)可初步判断,细胞膜对物质的转运具有      的特性。

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