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27. If you think that the illness might be serious, you should not    going to the doctor.

    A. put off         B. set about       C. hold back       D. give away

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26. Only ticket – holders were      to the stadium for the concept given by Jay Chou, so many of his fans were turned away.

    A. allowed        B. asked          C. agreed         D. admitted

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25. --- Jim managed to get into his house without the key.     ?

   ---I don’t know. He might have asked someone for help.

    A. What for        B. Guess how      C. So What        D. Who knows

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24. During your stay in Britain, you’ll face culture shock      language problems.

    A. as far as        B. as long as       C. as good as      D. as well as

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23. --- I try to memorize new words every day, but can only remember few of them.

   --- That’s not surprising. Newly – learnt words are easily forgotten unless frequently    

in communication.

    A. used           B. using          C. to use         D. be used

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22. It took the early settlers more than 150 years to build up     is now a modem city.

    A. where          B. which          C. what           D. that

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第一节:单项填空(共15小题,每小题1分,满分15分)

从A、B、C、D四个选项中,选出可以填入空白处的最佳选项。

21. --- Let’s discuss the question raised last night, shall we?

   --- There is no hurry for that. I     for a conference.

    A. headed         B. was heading     C. am heading     D. have headed

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31、(10分)某科研单位在调查湖水中细菌的污染情况时进行了实验。实验包括制备培养基、灭菌、接种及培养、菌落观察计数。

(1)培养基中含有蛋白胨、淀粉分别为细菌培养提供了___________________。对培养基进行灭菌,应该采用的方法是___________。接种时通常采用       方法,把聚集的菌种逐步分散到培养基的表面如果提高培养基中NaCl的浓度,可以用于筛选耐盐细菌,这种培养基被称为____________。

(2)该细菌所含的耐盐基因可通过酶切法获取,作为基因工程中的目的基因导入小麦中,其过程如右图所示。其中A为    ,耐盐基因的小麦细胞还应经过       ,在此过程中还将使用             两种植物激素,从而最终可获取含耐盐基因的小麦植株,当然这些小麦植株并不一定具有耐盐的特性,这是因为            

           。若在遗传过程中小麦植株丢失1条染色体,则不能产生正常配子而高度不育,则可用        处理幼芽,以便获得可育植株。

(3)图中C还可用于其他工程细菌中,若成功导入大肠杆菌内,则可能使大肠杆菌获得哪些特性?                    

贵州省晴隆一中2009年高中毕业班理科综合高考模拟试卷

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